Introduction: Primary maternal rubella virus infection during the first trimester of pregnancy carries a high risk for development of congenital rubella syndrome. World health organization estimates that worldwide more than 100,000 children are born with CRS each year, mostly in developing countries. Several guidelines suggest routine rubella serology testing in women trying for pregnancy. Objectives: This study was conducted to assess immunity (seroprevalence) to rubella among Indian women of childbearing age attending subfertility clinic. Materials and methods: Women attending subfertility clinic had serum sample tested to determine the seropositivity as part of subfertility work up.