Introduction: HIV/AIDS is the one of the biggest killers worldwide. An increasing number of women and their children are getting infected during delivery and breast feeding. Aim & Objectives: The study was designed to find out the prevalence of HIV/ AIDS in pregnant women of Eastern U.P. and Bihar. Epidemiological evaluation, of HIV positive antenatal patients, their course of disease and transmission to their babies were studied. Material & Methods: 7903 antenatal cases of Eastern U.P. and Bihar attending University Hospital, BHU (PPTCT Project UPSAC, NACO) were counseled and tested for HIV infection, 71 cases were found to be positive and were thoroughly evaluated in present study.